Literature summary extracted from
Furukawa, A.; Nagata, T.; Matsugami, A.; Habu, Y.; Sugiyama, R.; Hayashi, F.; Kobayashi, N.; Yokoyama, S.; Takaku, H.; Katahira, M.
Structure, interaction and real-time monitoring of the enzymatic reaction of wild-type APOBEC3G (2009), EMBO J., 28, 440-451.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.5.4.B9 |
expressed in Escherichia coli BL21(DE3/RIL) cells |
Homo sapiens |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.5.4.B9 |
F202A |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
F241K |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
L235A |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
L235K |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
L242A |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
L242K |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
T203A |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
V233A |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
V233K |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
3.5.4.B9 |
W232A |
the mutation causes a decrease of the enzyme activity |
Homo sapiens |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.5.4.B9 |
Homo sapiens |
Q9HC16 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.5.4.B9 |
Ni Sepharose resin column chromatography |
Homo sapiens |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.5.4.B9 |
cytosine in single-stranded viral DNA + H2O |
minus strand deamination occurs preferentially at a CCCA sequence. The third cytidine of the d(CCCA) segment is deaminated at an early stage and that then the second one is deaminated at a late stage, so the deamination is carried out in a strict 3'-5' order |
Homo sapiens |
uracil in single-stranded viral DNA + NH3 |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.5.4.B9 |
APOBEC3G |
- |
Homo sapiens |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.5.4.B9 |
physiological function |
the enzyme exhibits anti-human immunodeficiency virus-1 (HIV-1) activity by deaminating cytidines of the minus strand of HIV-1. Virus infectivity factor of HIV-1 counteracts the anti-HIV-1 activity of the enzyme |
Homo sapiens |